6B6L
The crystal structure of glycosyl hydrolase family 2 (GH2) member from Bacteroides cellulosilyticus DSM 14838
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.528, 227.135, 66.902 |
| Unit cell angles | 90.00, 108.83, 90.00 |
Refinement procedure
| Resolution | 34.881 - 2.000 |
| R-factor | 0.165 |
| Rwork | 0.163 |
| R-free | 0.20820 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.234 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.880 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.085 | 0.689 |
| Rmeas | 0.099 | 0.860 |
| Rpim | 0.051 | 0.508 |
| Number of reflections | 110119 | 5534 |
| <I/σ(I)> | 15.4 | 1.67 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 3.7 | 2.7 |
| CC(1/2) | 0.520 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.2M Potassium Chloride 20% (w/v) PEG3350 |
