6B66

IMPase (AF2372) R92Q/K164E with 400 mM Glutamate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2012-09-18
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.0
Spacegroup name	P 32
Unit cell lengths	89.906, 89.906, 100.935
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.645 - 2.500
R-factor	0.1591
Rwork	0.157
R-free	0.20130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lbv
RMSD bond length	0.030
RMSD bond angle	1.114
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.8_1069)

Data quality characteristics

	Overall
Low resolution limit [Å]	33.650
High resolution limit [Å]	2.500
Number of reflections	31585
<I/σ(I)>	2.925
Completeness [%]	99.8
Redundancy	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	100 mM myo inositiol 400 mM glutamate 18% PEG 3350 200 mM potassium sulfate 100 mM magnesium chloride 100 mM Hepes 7.0