6B63

IMPase (AF2372) with 25 mM Asp

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2012-09-06
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.0
Spacegroup name	P 32
Unit cell lengths	89.645, 89.645, 101.915
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.970 - 2.696
R-factor	0.1743
Rwork	0.172
R-free	0.21340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lbv
RMSD bond length	0.023
RMSD bond angle	1.187
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.8_1069)

Data quality characteristics

	Overall
Low resolution limit [Å]	33.970
High resolution limit [Å]	2.690
Number of reflections	25201
<I/σ(I)>	2.279
Completeness [%]	99.8
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	100 mM myo inositol 200 mM alpha diglycerol phosphate 4% PEG 3350 200 mM potassium sulfate 100 mM magnesium chloride 100 mM Hepes 7.0