6B62
IMPase (AF2372) with 400 mM Glutamate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | Cu FINE FOCUS |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-09-18 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 32 |
| Unit cell lengths | 89.509, 89.509, 103.139 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.750 - 2.003 |
| R-factor | 0.1617 |
| Rwork | 0.161 |
| R-free | 0.17150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lbv |
| RMSD bond length | 0.050 |
| RMSD bond angle | 1.223 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 44.750 |
| High resolution limit [Å] | 2.000 |
| Number of reflections | 62169 |
| <I/σ(I)> | 4.244 |
| Completeness [%] | 100.0 |
| Redundancy | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 100 mM myo inositol 400 mM Glutamate 25% PEG 3350 200 mM Potassium Sulfate 4 mM Magnesium Chloride |
