6B5R

Structure of PfCSP peptide 21 with human antibody CIS42

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-01-12
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	41.131, 70.570, 165.379
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.949 - 1.775
R-factor	0.193
Rwork	0.191
R-free	0.23710
RMSD bond length	0.006
RMSD bond angle	0.780
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall
Low resolution limit [Å]	26.949
High resolution limit [Å]	1.770
Number of reflections	35657
<I/σ(I)>	15
Completeness [%]	74.1
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	15% PEG3350, 9% Isopropanol, 0.12M Ammonium Citrate pH 8.5