6B5C

Structural Basis for Katanin Self-Assembly

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-28
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	40.100, 61.190, 117.620
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	58.810 - 2.400
R-factor	0.2171
Rwork	0.215
R-free	0.25310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6b5d
RMSD bond length	0.002
RMSD bond angle	0.502
Data reduction software	iMOSFLM
Data scaling software	SCALA (3.3.22)
Phasing software	PHASER
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	58.810	58.810	2.530
High resolution limit [Å]	2.400	7.590	2.400
Rmerge		0.054	0.569
Rmeas	0.109	0.061	0.697
Rpim	0.043	0.024	0.329
Total number of observations	73593	2646	7317
Number of reflections	11932	453	1713
<I/σ(I)>	10.5	21	2.1
Completeness [%]	99.9	99.8	99.4
Redundancy	6.2	5.8	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	0.1M TRIS pH 8.0 and 18% PEG 8000