6B3P
Crystal structure of CBMbc (family CBM26) from Eubacterium rectale Amy13K in Complex with Maltoheptaose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2015-07-31 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 134.200, 134.200, 231.130 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.150 - 2.010 |
| R-factor | 0.184 |
| Rwork | 0.184 |
| R-free | 0.19600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6b15 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.933 |
| Data reduction software | iMOSFLM (7.1.2) |
| Data scaling software | Aimless (0.5.8) |
| Phasing software | PHASER (2.5.6) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 28.150 |
| High resolution limit [Å] | 2.010 |
| Rmerge | 0.178 |
| Number of reflections | 81845 |
| <I/σ(I)> | 10.9 |
| Completeness [%] | 99.7 |
| Redundancy | 15.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 60% Tacsimate, 0.1 M Bis-Tris Propane, pH 7.0 |
