Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-07 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.2398 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.371, 97.012, 74.284 |
| Unit cell angles | 90.00, 100.69, 90.00 |
Refinement procedure
| Resolution | 45.566 - 1.770 |
| R-factor | 0.1871 |
| Rwork | 0.186 |
| R-free | 0.21870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5WF8 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.856 |
| Data reduction software | HKL-2000 (v715) |
| Data scaling software | HKL-2000 (v715) |
| Phasing software | PHASER (2.7.16) |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.800 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Rmerge | 0.069 | 0.326 |
| Rpim | 0.033 | 0.152 |
| Number of reflections | 57167 | 2821 |
| <I/σ(I)> | 36.2 | 5 |
| Completeness [%] | 90.9 | 91.1 |
| Redundancy | 5.1 | 5.6 |
| CC(1/2) | 0.972 | 0.976 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.5 | 293 | 20% PEG 3350, 10 mM MES pH 5.5 |
