6B29
Crystal structure of the second SH3 domain of STAC3 (309-364)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-08-26 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.979490 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 49.540, 57.960, 156.820 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.205 - 1.300 |
| R-factor | 0.1584 |
| Rwork | 0.157 |
| R-free | 0.18870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.223 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | REFMAC |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.205 | 1.350 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.052 | 0.371 |
| Number of reflections | 55375 | |
| <I/σ(I)> | 20.96 | 3.41 |
| Completeness [%] | 99.0 | 91.63 |
| Redundancy | 6.7 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 298 | 0.1M Bis-Tris, 22.5% PEG3350 and 0.2M ammonium acetate |
