6B25

Crystal structure of human STAC1 Tandem SH3 Domains (288-402)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-05-06
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97946
Spacegroup name	P 21 21 21
Unit cell lengths	28.958, 36.727, 113.001
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.500 - 2.390
R-factor	0.2054
Rwork	0.203
R-free	0.25880
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.528
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	56.500	35.000	2.460
High resolution limit [Å]	2.390	5.470	2.390
Rmerge	0.124	0.082	0.520
Rmeas	0.143	0.095	0.619
Rpim	0.069	0.046	0.327
Number of reflections	5120	496	406
<I/σ(I)>	7.4
Completeness [%]	98.0	97.8	93.8
Redundancy	4	3.8	3.2
CC(1/2)		0.986	0.793

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	298.15	0.1M sodium-HEPES, 25% PEG1000