6B14

Crystal structure of Spinach RNA aptamer in complex with Fab BL3-6S97N

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-11
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97921
Spacegroup name	C 1 2 1
Unit cell lengths	146.773, 79.993, 92.892
Unit cell angles	90.00, 111.76, 90.00

Refinement procedure

Resolution	49.540 - 1.640
R-factor	0.2124
Rwork	0.212
R-free	0.22680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4kze
RMSD bond length	0.005
RMSD bond angle	0.863
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.540	1.700
High resolution limit [Å]	1.640	1.640
Rmerge	0.043
Number of reflections	119527
<I/σ(I)>	12.6	0.7
Completeness [%]	97.9	97.09
Redundancy	3.4
CC(1/2)	0.996	0.996

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		296	0.1 M Succinic acid (pH 7.0), 12% Polyethylene glycol 3,350, room temperature.