6B0P

Zinc finger Domain of WT1(-KTS form) with 12+1mer Oligonucleotide with 3' Triplet GGT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2016-11-29
Detector	RAYONIX MX300-HS
Wavelength(s)	1.0000
Spacegroup name	P 21 21 2
Unit cell lengths	75.798, 84.402, 85.968
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.883 - 2.077
R-factor	0.1896
Rwork	0.188
R-free	0.22610
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.529
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.883	2.150
High resolution limit [Å]	2.077	2.080
Rmerge	0.124	0.922
Rpim	0.043	0.322
Number of reflections	33923	3354
<I/σ(I)>	17.5	2.9
Completeness [%]	100.0	100
Redundancy	9	8.8
CC(1/2)		0.851

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.6	289	20% PEG 3350 0.03M Citric Acid 0.07M BiS-TRIS Propane