6AZD
Crystal structure of Physcomitrella patens KAI2-like H
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-08-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.410, 52.650, 52.300 |
| Unit cell angles | 90.00, 95.51, 90.00 |
Refinement procedure
| Resolution | 37.018 - 1.970 |
| R-factor | 0.209706317639 |
| Rwork | 0.208 |
| R-free | 0.24288 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4jyp |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.605 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.100 | 2.040 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Rmerge | 0.167 | 0.445 |
| Rpim | 0.106 | 0.285 |
| Number of reflections | 18745 | 1833 |
| <I/σ(I)> | 8.17 | 3.85 |
| Completeness [%] | 98.3 | 97.66 |
| Redundancy | 3.5 | 3.4 |
| CC(1/2) | 0.982 | 0.549 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 1.8 M ammonium sulfate, 0.1 M MES 6.5, 5% PEG400 |
