6AZB

Crystal structure of Physcomitrella patens KAI2-like E

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-08-08
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.000
Spacegroup name	P 1 21 1
Unit cell lengths	46.860, 45.900, 146.550
Unit cell angles	90.00, 97.40, 90.00

Refinement procedure

Resolution	72.665 - 2.000
R-factor	0.22950736493
Rwork	0.221
R-free	0.25394
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4jyp
RMSD bond length	0.003
RMSD bond angle	0.606
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	72.670	2.072
High resolution limit [Å]	2.000	2.000
Rmerge	0.090	0.302
Rpim	0.055	0.185
Number of reflections	40640	3966
<I/σ(I)>	10.89	4.48
Completeness [%]	96.2	95.84
Redundancy	3.7	3.7
CC(1/2)	0.993	0.762

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	0.1 M Tris, pH 8.5, 10% PEG20000