6AYZ

Crystal structure of Asf1-Fab 12E complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-20
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979180
Spacegroup name	P 1 21 1
Unit cell lengths	86.811, 77.445, 101.734
Unit cell angles	90.00, 110.56, 90.00

Refinement procedure

Resolution	35.952 - 2.096
R-factor	0.1937
Rwork	0.191
R-free	0.23600
RMSD bond length	0.004
RMSD bond angle	0.666
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX ((1.10_2152: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	35.952
High resolution limit [Å]	2.096
Number of reflections	73866
<I/σ(I)>	32.2
Completeness [%]	99.4
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.1	298	0.2 M Ammonium Acetate, 20% PEG 3350