6AYL
Human adipocyte lipid-binding protein FABP4 in complex with fluorescein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.1271 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 33.190, 53.010, 74.990 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.490 - 1.860 |
| R-factor | 0.212 |
| Rwork | 0.211 |
| R-free | 0.23200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rzy |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.100 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.000 | 1.900 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Rmerge | 0.068 | 1.204 |
| Rpim | 0.042 | 0.921 |
| Number of reflections | 11207 | 501 |
| <I/σ(I)> | 13.9 | 0.9 |
| Completeness [%] | 96.3 | 73.9 |
| Redundancy | 6.3 | 4.4 |
| CC(1/2) | 0.999 | 0.391 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 1.6 M sodium citrate, pH 6.5 |
