6AY8

Cartilage homing cysteine-dense-peptides

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2017-03-22
Detector	RIGAKU SATURN 944
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	20.240, 34.927, 36.977
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.390 - 1.780
R-factor	0.11289
Rwork	0.110
R-free	0.16451
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6atm
RMSD bond length	0.018
RMSD bond angle	1.874
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.790
High resolution limit [Å]	1.760	1.760
Rmerge	0.078	0.145
Rpim	0.023	0.094
Number of reflections	2368	10
<I/σ(I)>	35.4	8.5
Completeness [%]	78.3	8.1
Redundancy	10.7	2.9
CC(1/2)		0.991

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.2M Ammonium sulfate, 20% PEG 3350