6AXU
W203Y Epi-isozizaene synthase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-11-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.540, 75.030, 107.570 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.515 - 1.822 |
| R-factor | 0.1966 |
| Rwork | 0.195 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kb9 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.698 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 37.520 |
| High resolution limit [Å] | 1.820 |
| Number of reflections | 33846 |
| <I/σ(I)> | 9.1 |
| Completeness [%] | 98.0 |
| Redundancy | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.2 M (NH4)2SO4, 0.1 M 2-(N-morpholino)ethanesulfonic acid monohydrate pH 6.5, 30% PEG monomethyl ether 5000 |
