6AXS

Structure of the V11I/T58A/P122A mutant of the HIV-1 capsid protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2016-12-19
Detector	RDI CMOS_8M
Wavelength(s)	1.000032
Spacegroup name	P 6
Unit cell lengths	92.475, 92.475, 57.675
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.076 - 2.402
R-factor	0.2306
Rwork	0.228
R-free	0.26590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	pdbid 4XFX
RMSD bond length	0.004
RMSD bond angle	0.633
Data reduction software	Aimless (0.5.32)
Data scaling software	Aimless (0.5.32)
Phasing software	PHASER (2.7.17)
Refinement software	PHENIX (1.11.1-2575_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	36.076	57.680	2.490
High resolution limit [Å]	2.400	8.990	2.400
Rmerge	0.078	0.040	0.781
Rmeas	0.082	0.042	0.829
Rpim	0.025	0.014	0.270
Total number of observations	114114
Number of reflections	10917	219	1140
<I/σ(I)>	20.7
Completeness [%]	98.2	94.5	98.1
Redundancy	10.5	9.9	8.8
CC(1/2)	0.998	0.997	0.815

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	PEG3350, NaI, Sodium cacodylate, Glycerol