6AX7

The crystal structure of a lysyl hydroxylase from Acanthamoeba polyphaga mimivirus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-15
Detector	RAYONIX MX-300
Wavelength(s)	0.98
Spacegroup name	P 43 21 2
Unit cell lengths	109.460, 109.460, 84.023
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.952 - 2.002
R-factor	0.1844
Rwork	0.183
R-free	0.21610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ax6
RMSD bond length	0.002
RMSD bond angle	0.509
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.952
High resolution limit [Å]	2.000	2.000
Rmerge	0.160
Rpim	0.079
Number of reflections	34800
<I/σ(I)>	8.82
Completeness [%]	99.6
Redundancy	4.9
CC(1/2)	0.993

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.29	289	400 nL drop containing 1:1 15 mg/mL L230 recombinant protein to 0.2 M potassium citrate, 20% PEG3350, 0.5% w/v n-dodecyl-N,N-dimethylamine-N-oxide