6AX6

The crystal structure of a lysyl hydroxylase from Acanthamoeba polyphaga mimivirus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-16
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.77
Spacegroup name	P 43 21 2
Unit cell lengths	109.210, 109.210, 83.910
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.490 - 2.241
R-factor	0.2246
Rwork	0.223
R-free	0.24040
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.499
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SHELXD
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.490
High resolution limit [Å]	2.240	2.240
Number of reflections	24748
<I/σ(I)>	23.95
Completeness [%]	97.7
Redundancy	23.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.29	289	0.2 M potassium citrate, 20% PEG3350, 4.0% v/v 2,2,2-trifluoroethanol