6AWZ
Structure of PR-10 allergen from peanut (Ara h 8.01).
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-20 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 59.869, 91.096, 55.079 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.900 |
| R-factor | 0.21603 |
| Rwork | 0.214 |
| R-free | 0.25649 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.720 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.043 | 0.379 |
| Rmeas | 0.057 | 0.566 |
| Rpim | 0.023 | 0.223 |
| Number of reflections | 23619 | 1090 |
| <I/σ(I)> | 41.6 | 3.3 |
| Completeness [%] | 98.7 | 93.3 |
| Redundancy | 6.3 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 20 % PEG 8000, 0.1 M Tris-HCl pH 8.5, 0.1 M MgCl2, 20 % PEG 400 |
