6AWF
Escherichia coli quinol:fumarate reductase crystallized without dicarboxylate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-21 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.13 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 113.318, 117.986, 125.291 |
| Unit cell angles | 90.00, 98.80, 90.00 |
Refinement procedure
| Resolution | 46.912 - 3.350 |
| R-factor | 0.2862 |
| Rwork | 0.285 |
| R-free | 0.31810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kf6 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.570 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.350 |
| Number of reflections | 42318 |
| <I/σ(I)> | 7.6 |
| Completeness [%] | 89.8 |
| Redundancy | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 15% PEG 550 MME, 25 mM CaCl2, 50 mM Bis-tris, pH 6.5 |
