6AW2
Crystal structure of the HopQ-CEACAM1 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-01-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979460 |
| Spacegroup name | P 2 2 21 |
| Unit cell lengths | 39.670, 103.077, 112.881 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 103.080 - 2.680 |
| R-factor | 0.23657 |
| Rwork | 0.234 |
| R-free | 0.27682 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5lp2 2gk2 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.028 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 103.080 | 2.810 |
| High resolution limit [Å] | 2.680 | 2.680 |
| Rmerge | 0.100 | 0.818 |
| Rpim | 0.079 | 0.664 |
| Number of reflections | 13386 | |
| <I/σ(I)> | 10.2 | 1.5 |
| Completeness [%] | 98.0 | 94.3 |
| Redundancy | 4.2 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 20% PEG 6000, 0.1M HEPES , pH 7.5, 0.2M NaCl |
