6AW1
Crystal structure of CEACAM3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-01-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979500 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 94.433, 94.433, 153.707 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 80.460 - 2.100 |
| R-factor | 0.20622 |
| Rwork | 0.205 |
| R-free | 0.22302 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qsq |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.521 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.460 | 2.160 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.057 | 1.022 |
| Rpim | 0.041 | 0.748 |
| Number of reflections | 20361 | |
| <I/σ(I)> | 16.7 | 1.8 |
| Completeness [%] | 98.9 | 99.3 |
| Redundancy | 5 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 1.0M Ammonium Sulphate, 1% PEG 3350, 0.1M Bis Tris pH 5.5 |
