6AVZ
Crystal structure of the HopQ-CEACAM3 WT complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-08-19 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.033200 |
| Spacegroup name | P 2 2 21 |
| Unit cell lengths | 39.479, 103.263, 112.565 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 103.260 - 2.050 |
| R-factor | 0.20519 |
| Rwork | 0.203 |
| R-free | 0.25027 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.098 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 103.260 | 2.110 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.897 | |
| Rpim | 0.043 | 0.719 |
| Number of reflections | 29535 | |
| <I/σ(I)> | 13.8 | 1.5 |
| Completeness [%] | 99.3 | 97.8 |
| Redundancy | 4.3 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 20% w/v PEG 3000, 0.1 M Bis-Tris-HCl, pH 6.5, 0.2 M calcium acetate, 1 % v/v glycerol |
