6AVW

Crystal structure of Arabidopsis thaliana SOBER1 L63A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-03-02
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	45.590, 51.550, 76.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.664 - 2.144
R-factor	0.189700656712
Rwork	0.188
R-free	0.22590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6avv
RMSD bond length	0.003
RMSD bond angle	0.580
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.10.1_2155))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.670	2.220
High resolution limit [Å]	2.140	2.140
Rmerge	0.143	0.513
Number of reflections	10296	1013
<I/σ(I)>	11.31	5.9
Completeness [%]	100.0
Redundancy	7.4	6.1
CC(1/2)	0.991	0.918

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	0.1 M HEPES pH 7.5, 2% (v/v) PEG 400, 2 M Ammonium sulfate.