6AVK

Streptavidin bound to peptide-like compound KPM-6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-08-16
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9774
Spacegroup name	I 2 2 2
Unit cell lengths	47.310, 93.520, 104.450
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	69.670 - 1.400
R-factor	0.174
Rwork	0.174
R-free	0.18600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vwa
RMSD bond length	0.010
RMSD bond angle	1.110
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	69.650	1.420
High resolution limit [Å]	1.400	1.400
Rmerge	0.042	0.337
Rmeas	0.050	0.416
Rpim	0.027	0.239
Number of reflections	45941	2212
<I/σ(I)>	18	3.4
Completeness [%]	100.0	98.3
Redundancy	5.8	4.5
CC(1/2)	0.999	0.861

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		290	0.2 M potassium iodide, 20% PEG3350