6AUF

Crystal structure of Metalo beta Lactamases MIM-1 from Novosphingobium pentaromativorans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2016-02-18
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 41 21 2
Unit cell lengths	67.961, 67.961, 216.670
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.981 - 2.603
R-factor	0.1775
Rwork	0.173
R-free	0.21940
RMSD bond length	0.006
RMSD bond angle	0.860
Data reduction software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	48.060
High resolution limit [Å]	2.600
Number of reflections	16486
<I/σ(I)>	21.8
Completeness [%]	99.9
Redundancy	14

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.05 M citrate, pH 5, 0.05 M Bis Tris Propane, pH 9.7, and 16% w/v PEG-3350