6AT7

Phenylalanine Ammonia-Lyase (PAL) from Sorghum bicolor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-01
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1
Spacegroup name	I 41 2 2
Unit cell lengths	126.304, 126.304, 337.477
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.655 - 2.493
R-factor	0.1616
Rwork	0.160
R-free	0.20180
RMSD bond length	0.003
RMSD bond angle	0.600
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall
Low resolution limit [Å]	44.655
High resolution limit [Å]	2.493
Rmerge	0.104
Number of reflections	49311
<I/σ(I)>	13.65
Completeness [%]	99.8
Redundancy	6.8
CC(1/2)	0.854

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	200 mM Ammonium formate pH 6.6, 20 % w/v polyethylene glycol 3,350