6ASN
E. coli phosphoenolpyruvate carboxykinase K212I F216V mutant bound to methanesulfonate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 12.3.1 |
| Synchrotron site | ALS |
| Beamline | 12.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-09-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.843, 75.605, 71.530 |
| Unit cell angles | 90.00, 94.69, 90.00 |
Refinement procedure
| Resolution | 47.304 - 1.549 |
| R-factor | 0.1748 |
| Rwork | 0.174 |
| R-free | 0.19310 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.973 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 47.304 |
| High resolution limit [Å] | 1.549 |
| Number of reflections | 168368 |
| <I/σ(I)> | 9.08 |
| Completeness [%] | 91.1 |
| Redundancy | 3.76 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.16 M ammonium sulfate, 0.08 M sodium acetate pH 4.8, 20% PEG 4000, 15% v/v glycerol |
