6ASL
Crystal Structure of Flavin monooxygenase CmoJ (earlier YtnJ) bound with FMN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-30 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.925 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 87.079, 87.079, 194.940 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.940 - 1.900 |
| R-factor | 0.173 |
| Rwork | 0.172 |
| R-free | 0.20100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yw1 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.952 |
| Data reduction software | iMOSFLM (7.2.1) |
| Phasing software | PHENIX ((1.10.1_2155: 000)) |
| Refinement software | PHENIX ((1.10.1_2155: 000)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.943 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.140 | 1.029 |
| Number of reflections | 59542 | |
| <I/σ(I)> | 16.14 | 1 |
| Completeness [%] | 100.0 | 99.83 |
| Redundancy | 6.8 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 50mM HEPES pH 7.5, 200 mM KCl, 29%-33% pentaerythritol propoxylate (5/4 PO/OH) |
