6ASI
E. coli phosphoenolpyruvate carboxykinase G209S mutant bound to methanesulfonate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 12.3.1 |
| Synchrotron site | ALS |
| Beamline | 12.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-04-02 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.008 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 125.310, 93.312, 46.525 |
| Unit cell angles | 90.00, 96.23, 90.00 |
Refinement procedure
| Resolution | 46.656 - 1.789 |
| R-factor | 0.1667 |
| Rwork | 0.166 |
| R-free | 0.18510 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.813 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 46.700 |
| High resolution limit [Å] | 1.789 |
| Number of reflections | 91394 |
| <I/σ(I)> | 18.39 |
| Completeness [%] | 92.4 |
| Redundancy | 2.23 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 16% PEG 3350, 0.1 M Bis-Tris pH 5.5, 0.3 M sodium chloride, 0.1 M sodium methanesulfonate |
