6ASB
CXXC and PHD-type zinc finger regions of FBXL19 in complex with DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-02-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.2831 |
| Spacegroup name | P 1 |
| Unit cell lengths | 55.729, 64.282, 67.324 |
| Unit cell angles | 103.53, 104.66, 99.40 |
Refinement procedure
| Resolution | 47.910 - 2.850 |
| R-factor | 0.215 |
| Rwork | 0.213 |
| R-free | 0.25100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | coordinates related to pdb entries 4HP3 4o64 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.060 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.8) |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 47.910 | 47.910 | 3.000 |
| High resolution limit [Å] | 2.850 | 9.010 | 2.850 |
| Rmerge | 0.100 | 0.064 | 0.655 |
| Rmeas | 0.117 | 0.076 | 0.764 |
| Rpim | 0.061 | 0.040 | 0.392 |
| Total number of observations | 71758 | 2168 | 10864 |
| Number of reflections | 19440 | ||
| <I/σ(I)> | 11.8 | 31.7 | 2.1 |
| Completeness [%] | 97.3 | 94.6 | 97.5 |
| Redundancy | 3.7 | 3.6 | 3.8 |
| CC(1/2) | 0.993 | 0.981 | 0.843 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 291 | 28% PEG2000-MME, 0.1 M Bis-Tris |
