6AR7
Crystal structure of a putative uncharacterized protein from Burkholderia thailandensis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-04-18 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 1 |
| Unit cell lengths | 48.690, 77.580, 80.860 |
| Unit cell angles | 65.46, 90.03, 83.51 |
Refinement procedure
| Resolution | 48.310 - 2.100 |
| R-factor | 0.172 |
| Rwork | 0.171 |
| R-free | 0.20600 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.842 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.7.17) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.311 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.076 | 0.542 |
| Number of reflections | 61171 | |
| <I/σ(I)> | 10.07 | 2.21 |
| Completeness [%] | 98.0 | 96.6 |
| Redundancy | 3.872 | 3.57 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.26 | 290 | 21.4 mg/mL ButhA.17069.a.A2.PS38198 (SeMet protein) against RigakuReagents JCSG+ A2 optimization screen (20.2% PEG3350, 100 mM sodium citrate tribasic / citric acid, pH 5.26) cryoprotectant: 20% glycerol (2 steps), tray 249240c7, puck pcm7-6 |
