6AR2

Structure of human SLMAP FHA domain in complex with pMST2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-13
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	38.758, 70.534, 91.015
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.239 - 1.550
R-factor	0.1627
Rwork	0.161
R-free	0.18770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ar0
RMSD bond length	0.009
RMSD bond angle	1.005
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.240	1.590
High resolution limit [Å]	1.550	1.550
Rpim	0.029	0.318
Number of reflections	36933	2719
<I/σ(I)>	39.4	1.4
Completeness [%]	99.8	97.3
Redundancy	12.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	200 mM KSCN, 20% PEG 3350