6AR1

Structure of a Thermostable Group II Intron Reverse Transcriptase with Template-Primer and Its Functional and Evolutionary Implications (RT/Duplex (Nat))

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	CCD
Collection date	2016-11-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9765
Spacegroup name	C 1 2 1
Unit cell lengths	179.151, 95.052, 71.567
Unit cell angles	90.00, 113.55, 90.00

Refinement procedure

Resolution	47.530 - 3.010
R-factor	0.2184
Rwork	0.216
R-free	0.25470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ar3
RMSD bond length	0.007
RMSD bond angle	1.170
Data reduction software	XDS (May 1, 2016)
Data scaling software	Aimless (0.5.31)
Phasing software	PHASER (2.7.16)
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.530	47.530	3.190
High resolution limit [Å]	3.010	9.030	3.010
Rmerge	0.113	0.023	0.852
Rmeas	0.121	0.025	0.914
Rpim	0.044	0.009	0.330
Total number of observations	166560
Number of reflections	21941	839	3497
<I/σ(I)>	16.2
Completeness [%]	99.8	99.2	99.1
Redundancy	7.6	7.3	7.6
CC(1/2)	0.999	1.000	0.886

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	Tris-HCl, sodium citrate tribasic dihydrate