6AQ6
X-ray crystal structure of Erythrina crista-galli lectin in complex with N-acetyllactosamine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-03-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 65 |
| Unit cell lengths | 134.669, 134.669, 81.212 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.081 - 1.903 |
| R-factor | 0.2209 |
| Rwork | 0.219 |
| R-free | 0.25460 |
| Starting model (for MR) | 1uzy |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.925 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.11_2567: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.081 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 65144 | |
| <I/σ(I)> | 38.4 | |
| Completeness [%] | 99.2 | |
| Redundancy | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | PEG3350, calcium acetate |
