6APP

Anti-Marburgvirus Nucleoprotein Single Domain Antibody A Complexed with Nucleoprotein C-terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2011-03-01
Detector	RIGAKU RAXIS HTC
Wavelength(s)	1.54178
Spacegroup name	P 21 21 21
Unit cell lengths	41.552, 46.150, 102.821
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.343 - 1.750
R-factor	0.1762
Rwork	0.172
R-free	0.21910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6apo
RMSD bond length	0.007
RMSD bond angle	1.090
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.150	1.840
High resolution limit [Å]	1.750	1.750
Rpim	0.039	0.281
Number of reflections	20487	2902
<I/σ(I)>	14.1	2.6
Completeness [%]	99.2	98.1
Redundancy	6.9	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	15% polyethylene glycol 6000, 5% glycerol