6APM

Hen egg-white lysozyme (WT), solved with serial millisecond crystallography using synchrotron radiation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	298
Detector technology	PIXEL
Collection date	2016-03-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 43 21 2
Unit cell lengths	79.035, 79.035, 38.245
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.500 - 2.050
R-factor	0.2081
Rwork	0.205
R-free	0.23320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4wm6
RMSD bond length	0.003
RMSD bond angle	0.703
Data reduction software	cctbx.xfel
Data scaling software	cctbx.prime
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.500	2.090
High resolution limit [Å]	2.050	2.050
Number of reflections	8025	390
<I/σ(I)>	5.54	1.19
Completeness [%]	100.0	100
Redundancy	29.1	19.6
CC(1/2)	0.900	0.480

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SMALL TUBES	4.5	298	2.5 M NaCl, 6% PEG 6K, 0.15 M sodium acetate (pH 4.5)