6AP4

Crystal structure of the DNA polymerase III subunit beta from Acinetobacter baumannii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-09-27
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.71073
Spacegroup name	P 1 21 1
Unit cell lengths	72.713, 328.597, 147.497
Unit cell angles	90.00, 91.53, 90.00

Refinement procedure

Resolution	164.300 - 2.950
R-factor	0.2503
Rwork	0.248
R-free	0.28670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4k3s
RMSD bond length	0.019
RMSD bond angle	2.522
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	164.300	50.000	3.060
High resolution limit [Å]	2.950	6.350	2.950
Rmerge	0.115	0.047	0.602
Rmeas	0.130	0.053	0.681
Rpim	0.059	0.024	0.315
Total number of observations	670605
Number of reflections	144290	14482	14388
<I/σ(I)>	8.4
Completeness [%]	99.8	99.3	99.9
Redundancy	4.6	4.6	4.6
CC(1/2)		0.995	0.786

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.6	294	0.1 M Tris-HCl pH 7.6, 0.1 M MgCl2, 7.5%(w/v) PEG 3350, 1% (v/v) DMSO