6ANJ
Synaptotagmin-7, C2A domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 65 |
| Unit cell lengths | 55.302, 55.302, 89.805 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.651 - 1.698 |
| R-factor | 0.148 |
| Rwork | 0.144 |
| R-free | 0.18110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2d8k |
| RMSD bond length | 0.017 |
| RMSD bond angle | 0.675 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.651 | 1.730 |
| High resolution limit [Å] | 1.698 | 1.700 |
| Rmerge | 0.054 | 0.985 |
| Rpim | 0.022 | 0.528 |
| Number of reflections | 17195 | |
| <I/σ(I)> | 34.6 | 1.42 |
| Completeness [%] | 99.7 | 99.5 |
| Redundancy | 6.6 | 4.1 |
| CC(1/2) | 0.542 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 21% t-butanol, 0.1 M Tris, 0.1 M calcium chloride, 0.125 M KCl, 15% ethylene glycol |
