6ANJ

Synaptotagmin-7, C2A domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-14
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97915
Spacegroup name	P 65
Unit cell lengths	55.302, 55.302, 89.805
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.651 - 1.698
R-factor	0.148
Rwork	0.144
R-free	0.18110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2d8k
RMSD bond length	0.017
RMSD bond angle	0.675
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.651	1.730
High resolution limit [Å]	1.698	1.700
Rmerge	0.054	0.985
Rpim	0.022	0.528
Number of reflections	17195
<I/σ(I)>	34.6	1.42
Completeness [%]	99.7	99.5
Redundancy	6.6	4.1
CC(1/2)		0.542

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	21% t-butanol, 0.1 M Tris, 0.1 M calcium chloride, 0.125 M KCl, 15% ethylene glycol