6AMS
Crystal structure of the DNA polymerase III subunit beta from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-01 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.95 |
| Spacegroup name | P 1 |
| Unit cell lengths | 68.498, 79.732, 86.302 |
| Unit cell angles | 63.43, 72.20, 87.08 |
Refinement procedure
| Resolution | 37.340 - 2.390 |
| R-factor | 0.2462 |
| Rwork | 0.244 |
| R-free | 0.28260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4k3s |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 37.340 | 50.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 6.510 | 2.400 |
| Rmerge | 0.108 | 0.033 | 0.587 |
| Total number of observations | 192222 | ||
| Number of reflections | 56764 | 2993 | 2760 |
| <I/σ(I)> | 9.3 | ||
| Completeness [%] | 94.0 | 99.1 | 90.2 |
| Redundancy | 3.4 | 3.8 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 294 | 0.1 M phosphate-citrate pH 6.0, 40% (w/v) ethanol, 5% (w/v) PEG 1000 |
