6AMQ
Crystal structure of the DNA polymerase III subunit beta from Enterobacter cloacae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-07-28 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 |
| Unit cell lengths | 66.320, 79.229, 81.218 |
| Unit cell angles | 110.98, 91.64, 89.96 |
Refinement procedure
| Resolution | 75.800 - 2.670 |
| R-factor | 0.249 |
| Rwork | 0.246 |
| R-free | 0.30040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4k3s |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 75.800 | 50.000 | 2.740 |
| High resolution limit [Å] | 2.690 | 7.290 | 2.690 |
| Rmerge | 0.111 | 0.052 | 0.626 |
| Total number of observations | 156941 | ||
| Number of reflections | 41701 | 2112 | 1933 |
| <I/σ(I)> | 9.5 | ||
| Completeness [%] | 96.1 | 97.3 | 86.7 |
| Redundancy | 3.8 | 3.8 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 294 | 0.1 M citrate pH 6.0, 0.15 M MgSO4, 10% (w/v) PEG 3350 |
