6AMN
Crystal Structure of Hsp104 N Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2001-03-27 |
| Detector | Brandeis B4 |
| Wavelength(s) | 1.10 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 179.096, 179.096, 69.710 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.526 - 2.816 |
| R-factor | 0.2136 |
| Rwork | 0.210 |
| R-free | 0.27890 |
| Structure solution method | MAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.399 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.530 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 14077 | 207 |
| <I/σ(I)> | 17.6 | |
| Completeness [%] | 84.2 | 12.7 |
| Redundancy | 14.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 277 | 33% PEG400, 50mM HEPES-HCl pH 7.2, 150mM Sodium formate |
