6AMK
Structure of Streptomyces venezuelae BldC-whiI opt complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-26 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 114.200, 114.200, 159.000 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 98.900 - 3.288 |
| R-factor | 0.2271 |
| Rwork | 0.223 |
| R-free | 0.26310 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.960 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 98.900 | 3.460 |
| High resolution limit [Å] | 3.280 | 3.280 |
| Rmeas | 0.140 | 1.590 |
| Rpim | 0.055 | 0.670 |
| Number of reflections | 16920 | |
| <I/σ(I)> | 11.7 | |
| Completeness [%] | 96.3 | 97.3 |
| Redundancy | 9.5 | 9.8 |
| CC(1/2) | 0.990 | 0.665 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 30% MPD, 0.1 M sodium acetate pH 5.0, 25% PEG 1500 |
