6AMA

Structure of S. coelicolor/S. venezuelae BldC-smeA-ssfA complex to 3.09 Angstrom

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-10-04
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 41 2 2
Unit cell lengths	159.351, 159.351, 130.829
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	101.116 - 3.090
R-factor	0.2146
Rwork	0.210
R-free	0.28130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6amk
RMSD bond length	0.003
RMSD bond angle	0.838
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX (1.6.4_486)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	101.116	3.260
High resolution limit [Å]	3.090	3.090
Rmeas	0.129
Rpim	0.051	0.905
Number of reflections	61611
<I/σ(I)>	8	1
Completeness [%]	100.0	100
Redundancy	3.2	3.1
CC(1/2)	0.997	0.350

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	35% PEG 400, 0.1 M CaCl2, 0.1 M HEPES 7.5