6ALR

VioC L-arginine hydroxylase bound to the vanadyl ion, L-arginine, and succinate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-03
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	80.586, 67.053, 62.840
Unit cell angles	90.00, 109.15, 90.00

Refinement procedure

Resolution	50.000 - 1.550
R-factor	0.1815
Rwork	0.180
R-free	0.20600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.086
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.580
High resolution limit [Å]	1.550	4.210	1.550
Rmerge	0.042	0.025	0.584
Rmeas	0.049	0.029	0.686
Rpim	0.025	0.015	0.356
Number of reflections	45909	2360	2287
<I/σ(I)>	11.9
Completeness [%]	100.0	99.8	100
Redundancy	3.7	3.7	3.6
CC(1/2)		0.999	0.802

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	1 M succinic acid, 0.1 M Tris pH 8.5