6ALQ

VioC L-arginine hydroxylase bound to Fe(II), L-arginine, and succinate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-28
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0331
Spacegroup name	C 1 2 1
Unit cell lengths	80.844, 66.751, 62.922
Unit cell angles	90.00, 109.27, 90.00

Refinement procedure

Resolution	29.097 - 1.670
R-factor	0.1817
Rwork	0.181
R-free	0.19960
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.130
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.097	29.097	1.570
High resolution limit [Å]	1.480	4.380	1.480
Rmerge	0.032	0.011	0.969
Rmeas	0.044	0.015	1.353
Total number of observations	150856
Number of reflections	36373	3781	6818
<I/σ(I)>	12.36	54.65	0.76
Completeness [%]	83.5	94.9	40.4
Redundancy	1.729	1.818	1.328
CC(1/2)	0.999	1.000	0.455

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	1.0 M succinic acid, 0.1 M Tris pH 8.5