6ALN

VioC L-arginine hydroxylase bound to Fe(II), 3S-hydroxy-L-arginine, and 2OG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2016-10-23
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	80.760, 66.653, 62.889
Unit cell angles	90.00, 109.21, 90.00

Refinement procedure

Resolution	50.000 - 1.800
R-factor	0.181
Rwork	0.179
R-free	0.21230
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.053
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.830
High resolution limit [Å]	1.800	4.880	1.800
Rmerge	0.053	0.020	0.578
Rmeas	0.062	0.024	0.680
Rpim	0.032	0.012	0.355
Total number of observations	109353
Number of reflections	29132	1513	1358
<I/σ(I)>	10.3
Completeness [%]	99.6	99.7	94.7
Redundancy	3.8	3.7	3.5
CC(1/2)		0.999	0.790

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	1 M 2-oxoglutarate, 0.1 M Tris pH 8.5